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magnetic beads  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec magnetic beads
    Magnetic Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 365 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd14+microbeads/CD14+MicroBeads%2C+human+-+lyophilized/pm42497858-391-9-12
    Average 98 stars, based on 365 article reviews
    magnetic beads - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Isolation:

    Article Title: Fisetin-mediated MYC restoration improves age-associated decline in macrophage function
    Article Snippet: .. Human monocytes were isolated by positive selection using CD14 microbeads (Miltenyi Biotec). .. Isolated monocytes were incubated in RPMI 1640 medium (Gibco) containing 10% (v/v) HI-FBS, 1% (v/v) L-Glutamine (Lonza), 1% (v/v) penicillin-streptomycin (Gibco) and 100 nM M-CSF (Peprotech) for 7-14 days at 37°C and 5% CO 2 .

    Article Title: GM-CSF downregulates type I IFN responses in glioblastoma-associated monocytes.
    Article Snippet: .. Primary human monocytes were isolated from PBMCs using magnetic activated cell sorting (MACS) with CD14 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Monocytes were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium, supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 10% fetal bovine serum (FBS) (Capricorn Scientific GmbH, Ebsdorfergrund, Germany) and 50 ng/ml recombinant human macrophage colonystimulating factor (M-CSF).

    Article Title: HIV-1 Tat protects macrophages from killing by the Exoenzyme U from Pseudomonas aeruginosa
    Article Snippet: .. Peripheral blood mononuclear cells were prepared by density gradient separation on Ficoll–Hypaque (Eurobio) and monocytes were isolated using CD14 microbeads (Miltenyi Biotec). .. Monocytes were differentiated in macrophages (MDMs) for 6–8 days in RPMI supplemented with 10 % FCS and 50 ng/ml of macrophage colony-stimulating factor (Immunotools).

    Article Title: HMGB1-mediated endoplasmic reticulum stress in monocyte-derived dendritic cells regulates CD4+ T cell immune responses in myasthenia gravis.
    Article Snippet: Fixation and permeabilization were performed using the Cytofix/Cytoperm Soln kit (Catalog No. 554715, BD Pharmingen, San Diego, USA). .. Intercellular proteins were stained with the IFN-α FITC (Clone RMMA1, Catalog No. 22100-3, PBL Assay Science, Piscataway, USA) 2.6 MoDC differentiation Human monocytes were positively isolated from fresh PBMCs with CD14 Microbeads (Catalog No. 130-050-201, Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Article Title: Human macrophage adhesion is significantly higher on SIBS based glaucoma microshunts than on common intraocular lens materials in vitro.
    Article Snippet: .. Thereafter, human MΦ were isolated from the PBMC suspension using CD14 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol. .. The magnetically labeled MΦ were subsequently positively isolated by passing the cell suspension through an LS Column placed in a MACS Separator (Miltenyi Biotec).

    Article Title: Chitin oligomers induce atypical NLRP3 inflammasome activation and innate immune training
    Article Snippet: .. Primary CD14 + monocytes were subsequently isolated from the total PBMC pool via positive selection using CD14 + MicroBeads (Cat. No. 130-050-201, Miltenyi Biotec) according to the manufacturer’s instructions. .. The isolated monocytes were resuspended in warm, serum-free RPMI-1640 medium supplemented with GlutaMAX (Cat. No. 61870036, Gibco) and 1% penicillin/streptomycin, seeded to allow for monocyte adherence, and the non-adherent supernatant was discarded.

    Article Title: Functional and dysfunctional T regulatory cell states in human tissues in RA and other autoimmune arthritic diseases.
    Article Snippet: .. Human CD14+ monocytes were isolated from PBMCs using CD14 MicroBeads (Miltenyi Biotec). .. Cells were differentiated into macrophages in RPMI 1640 supplemented with 10% heat-inactivated FBS (Atlanta Biologicals), 1 mM sodium pyruvate, 1 mM L-glutamine, non-essential amino acids, 2-mercaptoethanol and 5 mM HEPES (pH 7.4), in the presence of recombinant human macrophage colony-stimulating factor (M-CSF, 50 ng ml−1; PeproTech) for 3–4 days.

    Article Title: Glycosylated extracellular matrix drives immune suppression by modulating macrophage-T cell crosstalk in triple-negative breast cancer.
    Article Snippet: Isolation of monocytes from peripheral blood samples Peripheral blood mononuclear cells were isolated from leukocyte cones derived from healthy donors by centrifuging with Ficoll-Paque PLUS (GE Healthcare, 17-1440-03 AG) at 1400 × g for 20min with no deceleration. .. CD14+ monocytes were isolated using CD14 microbeads (Miltenyi, 130-050-201) in LS columns (Miltenyi, 130-042-401) and a QuadroMACS Separator (Miltenyi MACS, 13308). ..

    Selection:

    Article Title: Fisetin-mediated MYC restoration improves age-associated decline in macrophage function
    Article Snippet: .. Human monocytes were isolated by positive selection using CD14 microbeads (Miltenyi Biotec). .. Isolated monocytes were incubated in RPMI 1640 medium (Gibco) containing 10% (v/v) HI-FBS, 1% (v/v) L-Glutamine (Lonza), 1% (v/v) penicillin-streptomycin (Gibco) and 100 nM M-CSF (Peprotech) for 7-14 days at 37°C and 5% CO 2 .

    Article Title: Chitin oligomers induce atypical NLRP3 inflammasome activation and innate immune training
    Article Snippet: .. Primary CD14 + monocytes were subsequently isolated from the total PBMC pool via positive selection using CD14 + MicroBeads (Cat. No. 130-050-201, Miltenyi Biotec) according to the manufacturer’s instructions. .. The isolated monocytes were resuspended in warm, serum-free RPMI-1640 medium supplemented with GlutaMAX (Cat. No. 61870036, Gibco) and 1% penicillin/streptomycin, seeded to allow for monocyte adherence, and the non-adherent supernatant was discarded.

    FACS:

    Article Title: GM-CSF downregulates type I IFN responses in glioblastoma-associated monocytes.
    Article Snippet: .. Primary human monocytes were isolated from PBMCs using magnetic activated cell sorting (MACS) with CD14 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Monocytes were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium, supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 10% fetal bovine serum (FBS) (Capricorn Scientific GmbH, Ebsdorfergrund, Germany) and 50 ng/ml recombinant human macrophage colonystimulating factor (M-CSF).

    Magnetic Cell Separation:

    Article Title: GM-CSF downregulates type I IFN responses in glioblastoma-associated monocytes.
    Article Snippet: .. Primary human monocytes were isolated from PBMCs using magnetic activated cell sorting (MACS) with CD14 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany). .. Monocytes were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium, supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 10% fetal bovine serum (FBS) (Capricorn Scientific GmbH, Ebsdorfergrund, Germany) and 50 ng/ml recombinant human macrophage colonystimulating factor (M-CSF).

    Article Title: Glycosylated extracellular matrix drives immune suppression by modulating macrophage-T cell crosstalk in triple-negative breast cancer.
    Article Snippet: Isolation of monocytes from peripheral blood samples Peripheral blood mononuclear cells were isolated from leukocyte cones derived from healthy donors by centrifuging with Ficoll-Paque PLUS (GE Healthcare, 17-1440-03 AG) at 1400 × g for 20min with no deceleration. .. CD14+ monocytes were isolated using CD14 microbeads (Miltenyi, 130-050-201) in LS columns (Miltenyi, 130-042-401) and a QuadroMACS Separator (Miltenyi MACS, 13308). ..

    Staining:

    Article Title: HMGB1-mediated endoplasmic reticulum stress in monocyte-derived dendritic cells regulates CD4+ T cell immune responses in myasthenia gravis.
    Article Snippet: Fixation and permeabilization were performed using the Cytofix/Cytoperm Soln kit (Catalog No. 554715, BD Pharmingen, San Diego, USA). .. Intercellular proteins were stained with the IFN-α FITC (Clone RMMA1, Catalog No. 22100-3, PBL Assay Science, Piscataway, USA) 2.6 MoDC differentiation Human monocytes were positively isolated from fresh PBMCs with CD14 Microbeads (Catalog No. 130-050-201, Miltenyi Biotec, Bergisch Gladbach, Germany). ..

    Suspension:

    Article Title: Human macrophage adhesion is significantly higher on SIBS based glaucoma microshunts than on common intraocular lens materials in vitro.
    Article Snippet: .. Thereafter, human MΦ were isolated from the PBMC suspension using CD14 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol. .. The magnetically labeled MΦ were subsequently positively isolated by passing the cell suspension through an LS Column placed in a MACS Separator (Miltenyi Biotec).



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