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cd14 microbeads  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd14 microbeads
    Cd14 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 4699 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd14+microbeads/CD14+MicroBeads%2C+human/pm42855473-37-7-9
    Average 98 stars, based on 4699 article reviews
    cd14 microbeads - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: Developmental differences in platelet P-selectin expression modulate neonatal monocyte inflammatory responses.
    Article Snippet: .. The final pellet was then resuspended in 200–500 μL of PBS with 20–50 μL of CD14 microbeads (Miltenyi; #130- 050-201) and incubated for 15 min at 4°C. ..

    Isolation:

    Article Title: Differential effects of GM-CSF and M-CSF on long-interval LPS tolerance in human macrophages
    Article Snippet: .. CD14+ monocytes were isolated from donor blood (n = 6) by density gradient centrifugation using Ficoll solution [PanEco, Russia; density 1.077 g/ml], followed by immunomagnetic separation with CD14 MicroBeads (Miltenyi Biotec, Germany). ..

    Article Title: A Selective Pro‐Oxidant Drives Redox Reprogramming to Induce Ferroptosis and Immune Remodeling in Glioblastoma
    Article Snippet: .. CD14 + monocytes were isolated from peripheral blood mononuclear cells (PBMCs) using Ficoll‐Paque (GE Healthcare, Piscataway, NJ, USA) density‐gradient centrifugation followed by magnetic bead selection with CD14 MicroBeads (Miltenyi Biotec, Cologne, Germany) according to the manufacturer's protocol. ..

    Article Title: Targeting CD28 on T lineage malignancies with chimeric antigen receptor T cells.
    Article Snippet: .. CD14-positive monocytes were isolated from healthy-donor PBMCs using CD14 MicroBeads and LS columns (Miltenyi Biotec) according to the manufacturer’s instructions. .. Briefly, PBMCs were incubated with 20 μl MicroBeads per 1 × 107 cells for 15min at 4 °C, and CD14-positive cells were purified by magnetic separation.

    Gradient Centrifugation:

    Article Title: Differential effects of GM-CSF and M-CSF on long-interval LPS tolerance in human macrophages
    Article Snippet: .. CD14+ monocytes were isolated from donor blood (n = 6) by density gradient centrifugation using Ficoll solution [PanEco, Russia; density 1.077 g/ml], followed by immunomagnetic separation with CD14 MicroBeads (Miltenyi Biotec, Germany). ..

    Immunomagnetic Separation:

    Article Title: Differential effects of GM-CSF and M-CSF on long-interval LPS tolerance in human macrophages
    Article Snippet: .. CD14+ monocytes were isolated from donor blood (n = 6) by density gradient centrifugation using Ficoll solution [PanEco, Russia; density 1.077 g/ml], followed by immunomagnetic separation with CD14 MicroBeads (Miltenyi Biotec, Germany). ..

    Centrifugation:

    Article Title: A Selective Pro‐Oxidant Drives Redox Reprogramming to Induce Ferroptosis and Immune Remodeling in Glioblastoma
    Article Snippet: .. CD14 + monocytes were isolated from peripheral blood mononuclear cells (PBMCs) using Ficoll‐Paque (GE Healthcare, Piscataway, NJ, USA) density‐gradient centrifugation followed by magnetic bead selection with CD14 MicroBeads (Miltenyi Biotec, Cologne, Germany) according to the manufacturer's protocol. ..

    Selection:

    Article Title: A Selective Pro‐Oxidant Drives Redox Reprogramming to Induce Ferroptosis and Immune Remodeling in Glioblastoma
    Article Snippet: .. CD14 + monocytes were isolated from peripheral blood mononuclear cells (PBMCs) using Ficoll‐Paque (GE Healthcare, Piscataway, NJ, USA) density‐gradient centrifugation followed by magnetic bead selection with CD14 MicroBeads (Miltenyi Biotec, Cologne, Germany) according to the manufacturer's protocol. ..

    Purification:

    Article Title: Mitochondria transfer via tunneling nanotubes drives macrophage immunosuppression and metabolic reprogramming in pediatric AML.
    Article Snippet: Human monocyte-derived macrophages generation and polarization Peripheral blood mononuclear cells (PBMC) from healthy donors (Sanquin, Amsterdam, The Netherlands) were isolated by a densitygradient centrifugation using Lymphoprep (StemCell, Cologne, Germany). .. CD14+ monocytes were purified by MACS using CD14 MicroBeads (Miltenyi Biotec, Leiden, The Netherlands). .. Monocytes were differentiated into macrophages for 5 days in RPMI-1640 supplemented with 10% heat-inactivated human serum (SigmaAldrich, Zwijndrecht, The Netherlands) and 20 ng/mL M-CSF (PeproTech ThermoFisher, Breda, The Netherlands).

    Article Title: Antibody-opsonized extracellular traps modulate macrophage responses and attenuate their T cell-associated inflammatory and costimulatory characteristics
    Article Snippet: Buffy coats were diluted 1:1 in DPBS containing 2 mM EDTA, and peripheral blood mononuclear cells were isolated by Ficoll-Paque Plus density-gradient centrifugation (25 min, 800 x g), followed by one wash in DPBS containing 2 mM EDTA (centrifugation for 10 min, 300 x g) and two washes in DPBS containing 2 mM EDTA and 0.5% BSA (centrifugation for 10 min, 200 x g). .. Monocytes were purified using CD14 MicroBeads (Miltenyi, 130-097-052) according to the manufacturer’s instructions and resuspended in IMDM (Thermo Fisher Scientific, 12440061) supplemented with 1% penicillin-streptomycin, 1% FBS (Serena, S-FBS-SA-015), and 1% L-glutamine (monocyte buffer). .. Live cell counts were obtained using trypan blue with a Cellometer Auto T4 Bright Field Cell Counter, and monocyte purity was confirmed by flow cytometry as described below.

    Article Title: Helicobacter pylori -induced immune priming requires type I interferon signaling
    Article Snippet: Briefly, PBMC were isolated via density gradient centrifugation (836 × g, 20 min, RT) using Histopaque-1077 (Sigma-Aldrich, Vienna, Austria, #10771). .. Monocytes were subsequently purified using CD14 MicroBeads (Miltenyi Biotec, Bergisch-Gladbach, Germany, #130-050-201) following the manufacturer ́s instructions, with the exception that only 30% of the recommended amount of MicroBeads were used. ..

    Article Title: Inflammation-associated serum amyloid A amplifies amyloid-β-driven oxidative stress and microglial activation
    Article Snippet: Human monocyte-derived microglia-like cells (MDMi) generation MDMi cells were generated from peripheral blood mononuclear cells (PBMCs) isolated from healthy-donor buffy coats (Australian Red Cross Blood Service) by Lymphoprep (STEMCELL Technologies) densitygradient centrifugation [29]. .. CD14+ monocytes were purified by magnetic separation using CD14 MicroBeads (Miltenyi Biotec), seeded at 1 × 105 cells per well in 96-well plates, and differentiated under serum-free conditions in RPMI-1640 GlutaMAX (Gibco) supplemented with penicillin–streptomycin (Thermo Fisher Scientific), Fungizone (2.5 μg/mL; Life Technologies), and recombinant human cytokines: M-CSF (10 ng/mL), GM-CSF (10 ng/mL), NGF-β (10 ng/mL), MCP-1/CCL2 (100 ng/mL), and IL-34 (100 ng/mL; all PeproTech). ..

    Magnetic Cell Separation:

    Article Title: Mitochondria transfer via tunneling nanotubes drives macrophage immunosuppression and metabolic reprogramming in pediatric AML.
    Article Snippet: Human monocyte-derived macrophages generation and polarization Peripheral blood mononuclear cells (PBMC) from healthy donors (Sanquin, Amsterdam, The Netherlands) were isolated by a densitygradient centrifugation using Lymphoprep (StemCell, Cologne, Germany). .. CD14+ monocytes were purified by MACS using CD14 MicroBeads (Miltenyi Biotec, Leiden, The Netherlands). .. Monocytes were differentiated into macrophages for 5 days in RPMI-1640 supplemented with 10% heat-inactivated human serum (SigmaAldrich, Zwijndrecht, The Netherlands) and 20 ng/mL M-CSF (PeproTech ThermoFisher, Breda, The Netherlands).

    96 Well Plates:

    Article Title: Inflammation-associated serum amyloid A amplifies amyloid-β-driven oxidative stress and microglial activation
    Article Snippet: Human monocyte-derived microglia-like cells (MDMi) generation MDMi cells were generated from peripheral blood mononuclear cells (PBMCs) isolated from healthy-donor buffy coats (Australian Red Cross Blood Service) by Lymphoprep (STEMCELL Technologies) densitygradient centrifugation [29]. .. CD14+ monocytes were purified by magnetic separation using CD14 MicroBeads (Miltenyi Biotec), seeded at 1 × 105 cells per well in 96-well plates, and differentiated under serum-free conditions in RPMI-1640 GlutaMAX (Gibco) supplemented with penicillin–streptomycin (Thermo Fisher Scientific), Fungizone (2.5 μg/mL; Life Technologies), and recombinant human cytokines: M-CSF (10 ng/mL), GM-CSF (10 ng/mL), NGF-β (10 ng/mL), MCP-1/CCL2 (100 ng/mL), and IL-34 (100 ng/mL; all PeproTech). ..

    Recombinant:

    Article Title: Inflammation-associated serum amyloid A amplifies amyloid-β-driven oxidative stress and microglial activation
    Article Snippet: Human monocyte-derived microglia-like cells (MDMi) generation MDMi cells were generated from peripheral blood mononuclear cells (PBMCs) isolated from healthy-donor buffy coats (Australian Red Cross Blood Service) by Lymphoprep (STEMCELL Technologies) densitygradient centrifugation [29]. .. CD14+ monocytes were purified by magnetic separation using CD14 MicroBeads (Miltenyi Biotec), seeded at 1 × 105 cells per well in 96-well plates, and differentiated under serum-free conditions in RPMI-1640 GlutaMAX (Gibco) supplemented with penicillin–streptomycin (Thermo Fisher Scientific), Fungizone (2.5 μg/mL; Life Technologies), and recombinant human cytokines: M-CSF (10 ng/mL), GM-CSF (10 ng/mL), NGF-β (10 ng/mL), MCP-1/CCL2 (100 ng/mL), and IL-34 (100 ng/mL; all PeproTech). ..



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